human il 17c Search Results


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FIGURE 3. <t>IL-17C</t> expression is upregulated in autoimmune hepatitis. (A and B) WT mice were intravenously injected with 12 mg/kg Con A. (A) Relative mRNA expression of IL-17C at different time points in liver of Con A–treated WT mice, n = 5 for each group. (B and C) Representative image of liver sections from control and Con A–treated mice (B) or angioma and AIH patients (C) that were stained for IL-17C by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (D) Representative images of liver sections from control and Con A–treated mice (upper panel) or angioma and AIH patients (lower panel) that were stained for IL-17RE by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (E) IL-17C mRNA expression in different cell types isolated from liver of normal (n = 4) or Con A–treated mice (n = 6) at 8 h post injection. (F) IL-17C mRNA expression in liver of WTand Rag12/2 mice 8 h after Con A injection, n = 4–5 mice per group. (G) IL-17C mRNA expression in mouse primary hepatocytes treated for 4 h with IL-6 (20 ng/ml), IL-4 (20 ng/ml), IL-1a (10 ng/ml), IL-17A (50 ng/ml), IFN-g (20 ng/ml), IL-1b (10 ng/ml), TNF-a (10 ng/ml). Data shown are representative of two independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001.
Goat Anti Human Il 17c Polyclonal Ab, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 3. <t>IL-17C</t> expression is upregulated in autoimmune hepatitis. (A and B) WT mice were intravenously injected with 12 mg/kg Con A. (A) Relative mRNA expression of IL-17C at different time points in liver of Con A–treated WT mice, n = 5 for each group. (B and C) Representative image of liver sections from control and Con A–treated mice (B) or angioma and AIH patients (C) that were stained for IL-17C by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (D) Representative images of liver sections from control and Con A–treated mice (upper panel) or angioma and AIH patients (lower panel) that were stained for IL-17RE by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (E) IL-17C mRNA expression in different cell types isolated from liver of normal (n = 4) or Con A–treated mice (n = 6) at 8 h post injection. (F) IL-17C mRNA expression in liver of WTand Rag12/2 mice 8 h after Con A injection, n = 4–5 mice per group. (G) IL-17C mRNA expression in mouse primary hepatocytes treated for 4 h with IL-6 (20 ng/ml), IL-4 (20 ng/ml), IL-1a (10 ng/ml), IL-17A (50 ng/ml), IFN-g (20 ng/ml), IL-1b (10 ng/ml), TNF-a (10 ng/ml). Data shown are representative of two independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001.
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The effect of HVE and HT treatment (24 h) on the release of <t>IL-17C</t> ( a , b ) and MMP-9 ( c , d ) by HaCaT cells challenged by TNF-α (10 ng/mL), measured by the ELISA assay. HT was not effective until the highest concentration tested (10 μM corresponding to 4.93 μg/mL). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; reference inhibitor: E, epigallocatechin gallate (20 μM).
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The effect of HVE and HT treatment (24 h) on the release of <t>IL-17C</t> ( a , b ) and MMP-9 ( c , d ) by HaCaT cells challenged by TNF-α (10 ng/mL), measured by the ELISA assay. HT was not effective until the highest concentration tested (10 μM corresponding to 4.93 μg/mL). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; reference inhibitor: E, epigallocatechin gallate (20 μM).
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The effect of HVE and HT treatment (24 h) on the release of <t>IL-17C</t> ( a , b ) and MMP-9 ( c , d ) by HaCaT cells challenged by TNF-α (10 ng/mL), measured by the ELISA assay. HT was not effective until the highest concentration tested (10 μM corresponding to 4.93 μg/mL). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; reference inhibitor: E, epigallocatechin gallate (20 μM).
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The effect of HVE and HT treatment (24 h) on the release of <t>IL-17C</t> ( a , b ) and MMP-9 ( c , d ) by HaCaT cells challenged by TNF-α (10 ng/mL), measured by the ELISA assay. HT was not effective until the highest concentration tested (10 μM corresponding to 4.93 μg/mL). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; reference inhibitor: E, epigallocatechin gallate (20 μM).
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The effect of HVE and HT treatment (24 h) on the release of <t>IL-17C</t> ( a , b ) and MMP-9 ( c , d ) by HaCaT cells challenged by TNF-α (10 ng/mL), measured by the ELISA assay. HT was not effective until the highest concentration tested (10 μM corresponding to 4.93 μg/mL). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; reference inhibitor: E, epigallocatechin gallate (20 μM).
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MyBiosource Biotechnology il-35 human kit
The effect of HVE and HT treatment (24 h) on the release of <t>IL-17C</t> ( a , b ) and MMP-9 ( c , d ) by HaCaT cells challenged by TNF-α (10 ng/mL), measured by the ELISA assay. HT was not effective until the highest concentration tested (10 μM corresponding to 4.93 μg/mL). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; reference inhibitor: E, epigallocatechin gallate (20 μM).
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Image Search Results


FIGURE 3. IL-17C expression is upregulated in autoimmune hepatitis. (A and B) WT mice were intravenously injected with 12 mg/kg Con A. (A) Relative mRNA expression of IL-17C at different time points in liver of Con A–treated WT mice, n = 5 for each group. (B and C) Representative image of liver sections from control and Con A–treated mice (B) or angioma and AIH patients (C) that were stained for IL-17C by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (D) Representative images of liver sections from control and Con A–treated mice (upper panel) or angioma and AIH patients (lower panel) that were stained for IL-17RE by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (E) IL-17C mRNA expression in different cell types isolated from liver of normal (n = 4) or Con A–treated mice (n = 6) at 8 h post injection. (F) IL-17C mRNA expression in liver of WTand Rag12/2 mice 8 h after Con A injection, n = 4–5 mice per group. (G) IL-17C mRNA expression in mouse primary hepatocytes treated for 4 h with IL-6 (20 ng/ml), IL-4 (20 ng/ml), IL-1a (10 ng/ml), IL-17A (50 ng/ml), IFN-g (20 ng/ml), IL-1b (10 ng/ml), TNF-a (10 ng/ml). Data shown are representative of two independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis.

doi: 10.4049/jimmunol.1600977

Figure Lengend Snippet: FIGURE 3. IL-17C expression is upregulated in autoimmune hepatitis. (A and B) WT mice were intravenously injected with 12 mg/kg Con A. (A) Relative mRNA expression of IL-17C at different time points in liver of Con A–treated WT mice, n = 5 for each group. (B and C) Representative image of liver sections from control and Con A–treated mice (B) or angioma and AIH patients (C) that were stained for IL-17C by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (D) Representative images of liver sections from control and Con A–treated mice (upper panel) or angioma and AIH patients (lower panel) that were stained for IL-17RE by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (E) IL-17C mRNA expression in different cell types isolated from liver of normal (n = 4) or Con A–treated mice (n = 6) at 8 h post injection. (F) IL-17C mRNA expression in liver of WTand Rag12/2 mice 8 h after Con A injection, n = 4–5 mice per group. (G) IL-17C mRNA expression in mouse primary hepatocytes treated for 4 h with IL-6 (20 ng/ml), IL-4 (20 ng/ml), IL-1a (10 ng/ml), IL-17A (50 ng/ml), IFN-g (20 ng/ml), IL-1b (10 ng/ml), TNF-a (10 ng/ml). Data shown are representative of two independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001.

Article Snippet: Immunohistochemical staining was performed using a rabbit antimouse IL-17C polyclonal Ab (Bioss) or a goat anti-human IL-17C polyclonal Ab (R&D), rabbit anti-mouse/human IL-17RE polyclonal Ab (Biorbyt), and then incubated with a HRP-labeled secondary Ab and followed by 3,39-diaminobenzidine staining.

Techniques: Expressing, Injection, Control, Staining, Immunohistochemistry, Isolation

FIGURE 5. IL-17C is required for T and NK cell activation in Con A–induced hepatitis. WT and IL-17C2/2 mice were injected with 12 mg/kg Con A intravenously, and liver MNCs were isolated 24 h later. (A–F) Activation of liver CD4+

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis.

doi: 10.4049/jimmunol.1600977

Figure Lengend Snippet: FIGURE 5. IL-17C is required for T and NK cell activation in Con A–induced hepatitis. WT and IL-17C2/2 mice were injected with 12 mg/kg Con A intravenously, and liver MNCs were isolated 24 h later. (A–F) Activation of liver CD4+

Article Snippet: Immunohistochemical staining was performed using a rabbit antimouse IL-17C polyclonal Ab (Bioss) or a goat anti-human IL-17C polyclonal Ab (R&D), rabbit anti-mouse/human IL-17RE polyclonal Ab (Biorbyt), and then incubated with a HRP-labeled secondary Ab and followed by 3,39-diaminobenzidine staining.

Techniques: Activation Assay, Injection, Isolation

FIGURE 7. IL-17C–dependent liver damage re- quires NK cells. (A) Representative FACS of liver NK cells from WT (n = 7) and IL-17C2/2 (n = 9) mice 24 h after Con A treatment. (B) Real-time RT- PCR analysis of TRAIL mRNA level in sorted liver CD4+T, CD8+T, NK, NKT cells of WT and IL-17C2/2

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis.

doi: 10.4049/jimmunol.1600977

Figure Lengend Snippet: FIGURE 7. IL-17C–dependent liver damage re- quires NK cells. (A) Representative FACS of liver NK cells from WT (n = 7) and IL-17C2/2 (n = 9) mice 24 h after Con A treatment. (B) Real-time RT- PCR analysis of TRAIL mRNA level in sorted liver CD4+T, CD8+T, NK, NKT cells of WT and IL-17C2/2

Article Snippet: Immunohistochemical staining was performed using a rabbit antimouse IL-17C polyclonal Ab (Bioss) or a goat anti-human IL-17C polyclonal Ab (R&D), rabbit anti-mouse/human IL-17RE polyclonal Ab (Biorbyt), and then incubated with a HRP-labeled secondary Ab and followed by 3,39-diaminobenzidine staining.

Techniques: Quantitative RT-PCR

The effect of HVE and HT treatment (24 h) on the release of IL-17C ( a , b ) and MMP-9 ( c , d ) by HaCaT cells challenged by TNF-α (10 ng/mL), measured by the ELISA assay. HT was not effective until the highest concentration tested (10 μM corresponding to 4.93 μg/mL). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; reference inhibitor: E, epigallocatechin gallate (20 μM).

Journal: International Journal of Molecular Sciences

Article Title: Unveiling the Ability of Witch Hazel ( Hamamelis virginiana L.) Bark Extract to Impair Keratinocyte Inflammatory Cascade Typical of Atopic Eczema

doi: 10.3390/ijms23169279

Figure Lengend Snippet: The effect of HVE and HT treatment (24 h) on the release of IL-17C ( a , b ) and MMP-9 ( c , d ) by HaCaT cells challenged by TNF-α (10 ng/mL), measured by the ELISA assay. HT was not effective until the highest concentration tested (10 μM corresponding to 4.93 μg/mL). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; reference inhibitor: E, epigallocatechin gallate (20 μM).

Article Snippet: Coating anti-human IL-17C antibody and biotinylated anti-IL-17C were purchased from Novus (Novus biologicals, Bio-Techne s.r.l., Milan, Italy).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay