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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis.
doi: 10.4049/jimmunol.1600977
Figure Lengend Snippet: FIGURE 3. IL-17C expression is upregulated in autoimmune hepatitis. (A and B) WT mice were intravenously injected with 12 mg/kg Con A. (A) Relative mRNA expression of IL-17C at different time points in liver of Con A–treated WT mice, n = 5 for each group. (B and C) Representative image of liver sections from control and Con A–treated mice (B) or angioma and AIH patients (C) that were stained for IL-17C by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (D) Representative images of liver sections from control and Con A–treated mice (upper panel) or angioma and AIH patients (lower panel) that were stained for IL-17RE by immunohistochemistry. Scale bars, 100 mm (left and middle) and 50 mm (right), respectively. (E) IL-17C mRNA expression in different cell types isolated from liver of normal (n = 4) or Con A–treated mice (n = 6) at 8 h post injection. (F) IL-17C mRNA expression in liver of WTand Rag12/2 mice 8 h after Con A injection, n = 4–5 mice per group. (G) IL-17C mRNA expression in mouse primary hepatocytes treated for 4 h with IL-6 (20 ng/ml), IL-4 (20 ng/ml), IL-1a (10 ng/ml), IL-17A (50 ng/ml), IFN-g (20 ng/ml), IL-1b (10 ng/ml), TNF-a (10 ng/ml). Data shown are representative of two independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001.
Article Snippet: Immunohistochemical staining was performed using a rabbit antimouse IL-17C polyclonal Ab (Bioss) or a
Techniques: Expressing, Injection, Control, Staining, Immunohistochemistry, Isolation
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis.
doi: 10.4049/jimmunol.1600977
Figure Lengend Snippet: FIGURE 5. IL-17C is required for T and NK cell activation in Con A–induced hepatitis. WT and IL-17C2/2 mice were injected with 12 mg/kg Con A intravenously, and liver MNCs were isolated 24 h later. (A–F) Activation of liver CD4+
Article Snippet: Immunohistochemical staining was performed using a rabbit antimouse IL-17C polyclonal Ab (Bioss) or a
Techniques: Activation Assay, Injection, Isolation
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: IL-17C/IL-17RE Augments T Cell Function in Autoimmune Hepatitis.
doi: 10.4049/jimmunol.1600977
Figure Lengend Snippet: FIGURE 7. IL-17C–dependent liver damage re- quires NK cells. (A) Representative FACS of liver NK cells from WT (n = 7) and IL-17C2/2 (n = 9) mice 24 h after Con A treatment. (B) Real-time RT- PCR analysis of TRAIL mRNA level in sorted liver CD4+T, CD8+T, NK, NKT cells of WT and IL-17C2/2
Article Snippet: Immunohistochemical staining was performed using a rabbit antimouse IL-17C polyclonal Ab (Bioss) or a
Techniques: Quantitative RT-PCR
Journal: International Journal of Molecular Sciences
Article Title: Unveiling the Ability of Witch Hazel ( Hamamelis virginiana L.) Bark Extract to Impair Keratinocyte Inflammatory Cascade Typical of Atopic Eczema
doi: 10.3390/ijms23169279
Figure Lengend Snippet: The effect of HVE and HT treatment (24 h) on the release of IL-17C ( a , b ) and MMP-9 ( c , d ) by HaCaT cells challenged by TNF-α (10 ng/mL), measured by the ELISA assay. HT was not effective until the highest concentration tested (10 μM corresponding to 4.93 μg/mL). The data are expressed in percentages, relative to the stimulated control, which is arbitrarily assigned a value of 100%. ** p < 0.01, *** p < 0.001 versus stimulus. HVE, Hamamelis virginiana bark extract; reference inhibitor: E, epigallocatechin gallate (20 μM).
Article Snippet: Coating
Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay